BAIT

TAF3

TAF140, TAFII-140, TAFII140, RP11-379F12.5
TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
Homo sapiens
PREY

TBPL2

TBP2, TRF3
TATA box binding protein like 2
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Switching of the core transcription machinery during myogenesis.

Deato MD, Tjian R

Transcriptional mechanisms that govern cellular differentiation typically include sequence-specific DNA-binding proteins and chromatin-modifying activities. These regulatory factors are assumed necessary and sufficient to drive both divergent programs of proliferation and terminal differentiation. By contrast, potential contributions of the basal transcriptional apparatus to orchestrate cell-specific gene expression have been poorly explored. In order to probe alternative mechanisms that control differentiation, we ... [more]

Genes Dev. Sep. 01, 2007; 21(17);2137-49 [Pubmed: 17704303]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TAF3 TBPL2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Low-BioGRID
-

Curated By

  • BioGRID