BAIT

PORCN

2410004O13Rik, AW045557, DXHXS7465e, Mporc, Mporc-a, Mporc-b, Mporc-c, Mporc-d, Ppn, mMg61, porc, RP23-27I6.11-005
porcupine homolog (Drosophila)
GO Process (1)
GO Function (1)
GO Component (2)
Mus musculus
PREY

WNT7B

Wnt-7b
wingless-type MMTV integration site family, member 7B
GO Process (49)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family.

Tanaka K, Okabayashi K, Asashima M, Perrimon N, Kadowaki T

The Drosophila segment polarity gene product Porcupine (Porc) was first identified as being necessary for processing Wingless (Wg), a Drosophila Wnt (Wnt) family member. Mouse and Xenopus homologs of porc (Mporc and Xporc) were identified and found to encode endoplasmic reticulum (ER) proteins with multiple transmembrane domains. In contrast with porc, four different types of Mporc and Xporc mRNA (A-D) ... [more]

Eur. J. Biochem. Jul. 01, 2000; 267(13);4300-11 [Pubmed: 10866835]

Throughput

  • Low Throughput

Curated By

  • BioGRID