Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A, Bandyopadhyay S, Zofall M, Zhang K, Fischer T, Collins SR, Qu H, Shales M, Park HO, Hayles J, Hoe KL, Kim DU, Ideker T, Grewal SI, Weissman JS, Krogan NJ

An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway ... [more]

Science Oct. 17, 2008; 322(5900);405-10 [Pubmed: 18818364]

Quantitative Score

-16.0023 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.0 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SWD1 ARP42	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Ryan CJ (2012)	High	-7.1491	BioGRID	776204
ARP42 SWD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Ryan CJ (2012)	High	-7.1491	BioGRID	792115

Curated By

BioGRID

Interaction Summary

ARP42

Gene Ontology Biological Process

Gene Ontology Cellular Component

SWD1