An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Histone Demethylase JMJD2B Functions as a Co-Factor of Estrogen Receptor in Breast Cancer Proliferation and Mammary Gland Development.

Kawazu M, Saso K, Tong KI, McQuire T, Goto K, Son DO, Wakeham A, Miyagishi M, Mak TW, Okada H

Estrogen is a key regulator of normal function of female reproductive system and plays a pivotal role in the development and progression of breast cancer. Here, we demonstrate that JMJD2B (also known as KDM4B) constitutes a key component of the estrogen signaling pathway. JMJD2B is expressed in a high proportion of human breast tumors, and that expression levels significantly correlate ... [more]

PLoS ONE Mar. 30, 2011; 6(3);e17830 [Pubmed: 21445275]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KDM4C

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [IPI, ISO]chromatin binding [ISO]enzyme binding [IPI, ISO]histone demethylase activity [IDA]histone demethylase activity (H3-K9 specific) [ISO]

Gene Ontology Cellular Component

ARID1B