BAIT
RPS0B
NAB1B, YST2, ribosomal 40S subunit protein S0B, S2, S0B, L000002908, YLR048W
Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
GO Process (5)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- cytoplasmic translation [IMP]
- endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]
- endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]
- rRNA export from nucleus [IGI]
- ribosomal small subunit assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
SRS2
HPR5, DNA helicase SRS2, RADH1, RADH, L000000809, L000001578, YJL092W
DNA helicase and DNA-dependent ATPase; involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; functional homolog of human RTEL1
GO Process (6)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Srs2 overexpression reveals a helicase-independent role at replication forks that requires diverse cell functions.
Srs2 is a 3'-5' DNA helicase that regulates many aspects of DNA metabolism in Saccharomyces cerevisiae. It is best known for its ability to counteract homologous recombination by dismantling Rad51 filaments, but is also involved in checkpoint activation, adaptation and recovery, and in resolution of late recombination intermediates. To further address its biological roles and uncover new genetic interactions, we ... [more]
Unknown Mar. 31, 2011; 0(0); [Pubmed: 21459050]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: colony size (APO:0000063)
Additional Notes
- High Throughput: Three synthetic dosage lethality screens were carried out to identify genes required for cell viability upon overexpression of SRS2 and/or its helicase mutants srs2-K41A and srs2-K41R.
Curated By
- BioGRID