An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Proteomic analysis of SET-binding proteins.

Vera J, Estanyol JM, Canela N, Llorens F, Agell N, Itarte E, Bachs O, Jaumot M

The protein SET is involved in essential cell processes such as chromatin remodeling, apoptosis and cell cycle progression. It also plays a critical role in cell transformation and tumorogenesis. With the aim to study new SET functions we have developed a system to identify SET-binding proteins by combining affinity chromatography, MS, and functional studies. We prepared SET affinity chromatography columns ... [more]

Proteomics Feb. 01, 2007; 7(4);578-87 [Pubmed: 17309103]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SET CSNK2A1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Vera J (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SET

Gene Ontology Biological Process

Gene Ontology Cellular Component

CSNK2A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-catenin binding [IDA]kinase activity [IDA]protein N-terminus binding [ISO]protein binding [IPI]protein phosphatase regulator activity [IDA]protein serine/threonine kinase activity [ISO]