An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of novel functional TBP-binding sites and general factor repertoires.

Denissov S, van Driel M, Voit R, Hekkelman M, Hulsen T, Hernandez N, Grummt I, Wehrens R, Stunnenberg H

Our current knowledge of the general factor requirement in transcription by the three mammalian RNA polymerases is based on a small number of model promoters. Here, we present a comprehensive chromatin immunoprecipitation (ChIP)-on-chip analysis for 28 transcription factors on a large set of known and novel TATA-binding protein (TBP)-binding sites experimentally identified via ChIP cloning. A large fraction of identified ... [more]

EMBO J. Feb. 21, 2007; 26(4);944-54 [Pubmed: 17268553]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TAF12

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]histone acetyltransferase activity [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA]transcription coactivator activity [IDA]transcription factor binding [IDA]

Gene Ontology Cellular Component

TAF1A