CRY1
Gene Ontology Biological Process
- anthocyanin-containing compound metabolic process [IMP]
- blue light signaling pathway [TAS]
- circadian regulation of calcium ion oscillation [IMP]
- circadian rhythm [NAS]
- detection of light stimulus [IMP]
- oxidation-reduction process [IMP]
- photomorphogenesis [IMP]
- protein autophosphorylation [IDA]
- regulation of meristem growth [IGI]
- regulation of unidimensional cell growth [IMP]
- response to blue light [IMP, NAS]
- response to water deprivation [IGI]
- singlet oxygen-mediated programmed cell death [IMP]
- stomatal movement [IGI]
Gene Ontology Molecular Function
SPA1
Gene Ontology Biological Process
- chloroplast organization [IMP]
- negative regulation of photomorphogenesis [IMP]
- photomorphogenesis [IMP]
- red or far-red light signaling pathway [IMP]
- red, far-red light phototransduction [IGI]
- regulation of photoperiodism, flowering [IMP]
- response to far red light [IEP]
- response to red light [IEP]
- short-day photoperiodism, flowering [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light.
Plant photoreceptors mediate light suppression of the E3 ubiquitin ligase COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) to affect gene expression and photomorphogenesis. However, how photoreceptors mediate light regulation of COP1 activity remains unknown. We report here that Arabidopsis blue-light receptor cryptochrome 1 (CRY1) undergoes blue-light-dependent interaction with the COP1-interacting protein SPA1 (SUPPRESSOR OF PHYTOCHROME A). We further show that the CRY1-SPA1 interaction ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CRY1 SPA1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SPA1 CRY1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CRY1 SPA1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CRY1 SPA1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID