BAIT

DNA2

WEB2, bifuctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2, L000003158, YHR164C
Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair and processing of meiotic DNA double strand breaks; required for normal life span; component of telomeric chromatin, with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

MRC1

YCL060C, chromatin-modulating protein MRC1, YCL061C
S-phase checkpoint protein required for DNA replication; couples DNA helicase and DNA polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; with Hog1p defines a novel S-phase checkpoint that permits eukaryotic cells to prevent conflicts between DNA replication and transcription; protects uncapped telomeres; degradation via Dia2p help cells resume cell cycle
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Inviability of a DNA2 deletion mutant is due to the DNA damage checkpoint.

Budd ME, Antoshechkin IA, Reis C, Wold BJ, Campbell JL

Dna2 is a dual polarity exo/endonuclease, and 5' to 3' DNA helicase involved in Okazaki Fragment Processing (OFP) and Double Strand Break (DSB) Repair. In yeast, DNA2 is an essential gene, as expected for a DNA replication protein. Suppression of the lethality of dna2D mutants has been found to occur by two mechanisms, overexpression of RAD27 (scFEN1) , encoding a 5' to ... [more]

Unknown May. 15, 2011; 10(10); [Pubmed: 21508669]

Throughput

  • Low Throughput

Ontology Terms

  • viability (APO:0000111)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MRC1 DNA2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4536BioGRID
2030943
DNA2 MRC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2264BioGRID
1988597
DNA2 MRC1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
206174

Curated By

  • BioGRID