Golgi Dsc E3 ligase complex subunit Dsc1
GO Process (3)
GO Function (1)
GO Component (2)
Schizosaccharomyces pombe (972h)


ucp13, SPAC2C4.15c
UBX domain protein Ubx2
GO Process (2)
GO Function (2)
GO Component (2)
Schizosaccharomyces pombe (972h)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.


Yeast SREBP Cleavage Activation Requires the Golgi Dsc E3 Ligase Complex.

Stewart EV, Nwosu CC, Tong Z, Roguev A, Cummins TD, Kim DU, Hayles J, Park HO, Hoe KL, Powell DW, Krogan NJ, Espenshade PJ

Mammalian lipid homeostasis requires proteolytic activation of membrane-bound sterol regulatory element binding protein (SREBP) transcription factors through sequential action of the Golgi Site-1 and Site-2 proteases. Here we report that while SREBP function is conserved in fungi, fission yeast employs a different mechanism for SREBP cleavage. Using genetics and biochemistry, we identified four genes defective for SREBP cleavage, dsc1-4, encoding ... [more]

Mol. Cell Apr. 22, 2011; 42(2);160-71 [Pubmed: 21504829]

Quantitative Score

  • -2.956183 [SGA Score]


  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).

Curated By

  • BioGRID