BAIT
DSC3
SPAC20H4.02
Golgi Dsc E3 ligase complex subunit Dsc3
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
PREY
RAD50
SPAP4C9.01c, SPAC1556.01c
DNA repair protein Rad50
GO Process (10)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- DNA duplex unwinding [TAS]
- double-strand break repair via homologous recombination [IGI]
- double-strand break repair via nonhomologous end joining [IMP]
- gene conversion at mating-type locus, DNA repair synthesis [IMP]
- homologous recombination-dependent replication fork processing [IMP]
- intra-S DNA damage checkpoint [IMP]
- meiotic DNA double-strand break formation [IMP]
- mitotic DNA damage checkpoint [IMP]
- reciprocal meiotic recombination [IMP]
- telomere maintenance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Schizosaccharomyces pombe (972h)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Yeast SREBP Cleavage Activation Requires the Golgi Dsc E3 Ligase Complex.
Mammalian lipid homeostasis requires proteolytic activation of membrane-bound sterol regulatory element binding protein (SREBP) transcription factors through sequential action of the Golgi Site-1 and Site-2 proteases. Here we report that while SREBP function is conserved in fungi, fission yeast employs a different mechanism for SREBP cleavage. Using genetics and biochemistry, we identified four genes defective for SREBP cleavage, dsc1-4, encoding ... [more]
Mol. Cell Apr. 22, 2011; 42(2);160-71 [Pubmed: 21504829]
Quantitative Score
- -8.091789 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID