BAIT

CALB1

CALB
calbindin 1, 28kDa
GO Process (2)
GO Function (3)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

CACNA1D

CACH3, CACN4, CACNL1A2, CCHL1A2, Cav1.3, PASNA, SANDD
calcium channel, voltage-dependent, L type, alpha 1D subunit
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

New insights into the function and regulation of vitamin D target proteins.

Christakos S, Dhawan P, Peng X, Obukhov AG, Nowycky MC, Benn BS, Zhong Y, Liu Y, Shen Q

Calbindin-D(28k) has been reported to be a facilitator of calcium diffusion and to protect against apoptotic cell death. Most recently, we found that the presence of calbindin-D(28k) results in reduced calcium influx through voltage-dependent L-type Ca(2+) channels and enhanced sensitivity of the channels to calcium dependent inactivation. Co-immunoprecipitation and GST pull down assays indicate that calbindin-D(28k) interacts with the C-terminus ... [more]

J. Steroid Biochem. Mol. Biol. Mar. 01, 2007; 103(3);405-10 [Pubmed: 17257825]

Throughput

  • Low Throughput

Curated By

  • BioGRID