A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

An E3 ligase complex regulates SET-domain polycomb group protein activity in Arabidopsis thaliana.

Jeong CW, Roh H, Dang TV, Choi YD, Fischer RL, Lee JS, Choi Y

Transcriptional repression via methylation of histone H3 lysine 27 (H3K27) by the polycomb repressive complex 2 (PRC2) is conserved in higher eukaryotes. The Arabidopsis PRC2 controls homeotic gene expression, flowering time, and gene imprinting. Although downstream target genes and the regulatory mechanism of PRC2 are well understood, much less is known about the significance of posttranslational regulation of PRC2 protein ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 10, 2011; 108(19);8036-41 [Pubmed: 21518870]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT1G65740 CLF	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Jeong CW (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT1G65740

Gene Ontology Cellular Component

CLF

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS]single-stranded RNA binding [IDA]