BAIT
ROX1
REO1, L000001661, YPR065W
Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
GO Process (2)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding, bending [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IMP]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- DNA binding, bending [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IMP]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CTF18
CHL12, L000000431, L000000325, YMR078C
Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
GO Process (4)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Rewiring of genetic networks in response to DNA damage.
Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They ... [more]
Science Dec. 03, 2010; 330(6009);1385-9 [Pubmed: 21127252]
Quantitative Score
- -2.537708 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants in MMS-treated conditions. Genetic interactions were considered significant if they had an S score >=2.0 for positive interactions (epistatic or suppressor interactions) and S score <=2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID