Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of differential protein interactors of lamin A and progerin.

Kubben N, Voncken JW, Demmers J, Calis C, van Almen G, Pinto Y, Misteli T

The nuclear lamina is an interconnected meshwork of intermediate filament proteins underlying the nuclear envelope. The lamina is an important regulator of nuclear structural integrity as well as nuclear processes, including transcription, DNA replication and chromatin remodeling. The major components of the lamina are A- and B-type lamins. Mutations in lamins impair lamina functions and cause a set of highly ... [more]

Unknown Dec. 01, 2009; 1(6);513-525 [Pubmed: 21327095]

Throughput

  • Low Throughput

Curated By

  • BioGRID