BAIT

DMC1

ISC2, recombinase DMC1, L000000509, YER179W
Meiosis-specific recombinase required for repair of double-strand breaks; also required for pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p
GO Process (3)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

LCD1

DDC2, PIE1, YDR499W
Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates.

Refolio E, Cavero S, Marcon E, Freire R, San-Segundo PA

During meiosis, accurate segregation of intact chromosomes is essential for generating healthy gametes. Defects in recombination and/or chromosome synapsis activate the pachytene checkpoint, which delays meiotic cell cycle progression to avoid aberrant chromosome segregation and formation of defective gametes. Here, we characterize the role of the conserved DNA damage checkpoint protein Ddc2/ATRIP in this meiotic surveillance mechanism. We show that ... [more]

J. Cell. Sci. Jul. 15, 2011; 124(0);2488-500 [Pubmed: 21693576]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell cycle progression (APO:0000253)
  • phenotype: meiosis (APO:0000039)
  • phenotype: protein/peptide modification (APO:0000131)

Additional Notes

  • Dmc1 mutants display a robust checkpoint delay that is alleviated in by deletion of ddc2
  • deletion of ddc2 alleviates the meiotic block seen in a rad54/dmc1 double mutant background
  • deltion of ddc2 also blocks the phosphorylation of mek1
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DMC1 LCD1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
545184
DMC1 LCD1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
545188

Curated By

  • BioGRID