An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The SH3 domain of alphaII spectrin is a target for the Fanconi anemia protein, FANCG.

Lefferts JA, Wang C, Sridharan D, Baralt M, Lambert MW

The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is dependent on FA proteins, acts as a scaffold to ... [more]

Biochemistry Jan. 20, 2009; 48(2);254-63 [Pubmed: 19102630]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SPTAN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

FANCF