BAIT

NRD1

Nrd1 complex RNA-binding subunit, L000002862, YNL251C
RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size
Saccharomyces cerevisiae (S288c)
PREY

PHO23

L000004300, YNL097C
Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway.

Terzi N, Churchman LS, Vasiljeva L, Weissman J, Buratowski S

In Saccharomyces cerevisiae, the Nrd1-Nab3-Sen1 pathway mediates termination of snoRNAs and cryptic unstable transcripts (CUTs). Both Nrd1 and the Set1 histone H3K4 methyltransferase complex interact with the RNA polymerase II (Pol II) during early elongation, leading us to test whether these two processes are functionally linked. Deletion of SET1 exacerbates the growth rate and termination defects of nrd1 mutants. Set1 ... [more]

Unknown Jun. 27, 2011; 0(0); [Pubmed: 21709022]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: rna accumulation (APO:0000224)

Additional Notes

  • double mutants show decreased growth and increased read-through of snRNA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NRD1 PHO23
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.016BioGRID
217561
NRD1 PHO23
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1259BioGRID
408331

Curated By

  • BioGRID