BAIT

PHO23

L000004300, YNL097C
Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3
Saccharomyces cerevisiae (S288c)
PREY

RPB2

RPB150, RPO22, SIT2, SOH2, DNA-directed RNA polymerase II core subunit RPB2, B150, L000001588, L000001676, YOR151C
RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
GO Process (1)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway.

Terzi N, Churchman LS, Vasiljeva L, Weissman J, Buratowski S

In Saccharomyces cerevisiae, the Nrd1-Nab3-Sen1 pathway mediates termination of snoRNAs and cryptic unstable transcripts (CUTs). Both Nrd1 and the Set1 histone H3K4 methyltransferase complex interact with the RNA polymerase II (Pol II) during early elongation, leading us to test whether these two processes are functionally linked. Deletion of SET1 exacerbates the growth rate and termination defects of nrd1 mutants. Set1 ... [more]

Unknown Jun. 27, 2011; 0(0); [Pubmed: 21709022]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: rna accumulation (APO:0000224)

Additional Notes

  • genetic complex
  • nrd1/set1/rpb2 and nrd1/pho23/rpb2 triple mutants show decreased growth and increased snRNA read-through

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHO23 RPB2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1668BioGRID
2064953

Curated By

  • BioGRID