An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The 14-3-3 isoforms chi and epsilon differentially bind client proteins from developing Arabidopsis seed.

Swatek KN, Graham K, Agrawal GK, Thelen JJ

The 14-3-3-protein family is prominently expressed during seed filling and modulates protein interactions and enzymatic activities, in a phosphorylation-dependent manner. To investigate the role(s) of 14-3-3 proteins in oilseed development, we have begun to characterize the Arabidopsis thaliana 14-3-3 "interactome" for two phylogenetically distinct isoforms. Proteins from developing Arabidopsis seed were incubated with a Sepharoseâ„¢ affinity matrix containing covalently bound ... [more]

Unknown Jul. 18, 2011; 0(0); [Pubmed: 21766784]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GRF1 GF14 PHI	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Shin R (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GRF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein phosphorylated amino acid binding [TAS]

Gene Ontology Cellular Component

GF14 PHI