BAIT

GRF10

14-3-3 PROTEIN G-BOX FACTOR14 EPSILON, 14-3-3EPSILON, GF14 EPSILON, general regulatory factor 10, AT1G22300
14-3-3-like protein GF14 epsilon
GO Process (2)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CINV1

F15O4.33, cytosolic invertase 1, AT1G35580
alkaline/neutral invertase CINV1
Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The 14-3-3 isoforms chi and epsilon differentially bind client proteins from developing Arabidopsis seed.

Swatek KN, Graham K, Agrawal GK, Thelen JJ

The 14-3-3-protein family is prominently expressed during seed filling and modulates protein interactions and enzymatic activities, in a phosphorylation-dependent manner. To investigate the role(s) of 14-3-3 proteins in oilseed development, we have begun to characterize the Arabidopsis thaliana 14-3-3 "interactome" for two phylogenetically distinct isoforms. Proteins from developing Arabidopsis seed were incubated with a Sepharoseâ„¢ affinity matrix containing covalently bound ... [more]

Unknown Jul. 18, 2011; 0(0); [Pubmed: 21766784]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CINV1 GRF10
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID