BAIT

RPS2

DISEASE RESISTANCE PROTEIN RPS2, F20B18.200, F20B18_200, RESISTANT TO P. SYRINGAE 2, AT4G26090
disease resistance protein RPS2
GO Process (4)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AT3G01290

T22N4.8, T22N4_8
SPFH/Band 7/PHB domain-containing membrane-associated protein
GO Process (1)
GO Function (0)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Physical association of Arabidopsis hypersensitive induced reaction proteins (HIRs) with the immune receptor RPS2.

Qi Y, Tsuda K, Nguyen LV, Wang X, Lin J, Murphy AS, Glazebrook J, Thordal-Christensen H, Katagiri F

Arabidopsis RPS2 is a typical NB-LRR resistance (R) protein, which indirectly recognizes the bacterial effector protein AvrRpt2 and thereby activates effector-triggered immunity (ETI). Previously, we identified two hypersensitive induced reaction (AtHIR) proteins, AtHIR1 (At1g09840) and AtHIR2 (At3g01290) as potential RPS2 complex components. AtHIR proteins contain the stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (also known as the prohibitin (PHB) domain or band 7 domain). ... [more]

Unknown Jul. 13, 2011; 0(0); [Pubmed: 21757708]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPS2 AT3G01290
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
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Curated By

  • BioGRID