BAIT

RPB4

CTF15, DNA-directed RNA polymerase II subunit RPB4, B32, L000001678, YJL140W

RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

GO Process (6)

GO Function (4)

GO Component (4)

Gene Ontology Biological Process

mRNA export from nucleus in response to heat stress [IMP]

nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]

positive regulation of translational initiation [IMP]

recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]

transcription from RNA polymerase II promoter [IMP]

transcription initiation from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IDA]
translation initiation factor binding [IPI]

Gene Ontology Cellular Component

DNA-directed RNA polymerase II, core complex [IDA]

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPO21

RPB1, RPB220, SUA8, DNA-directed RNA polymerase II core subunit RPO21, B220, L000001744, YDL140C

RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime

GO Process (2)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

transcription from RNA polymerase II promoter [IMP]

translesion synthesis [IMP]

Gene Ontology Molecular Function

RNA polymerase II activity [IDA]
RNA-directed RNA polymerase activity [IDA]

Gene Ontology Cellular Component

DNA-directed RNA polymerase II, core complex [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases.

Ruprich-Robert G, Wery M, Despres D, Boulard Y, Thuriaux P

The catalytic center of yeast RNA polymerase II and III contains an acidic loop borne by their second largest subunit (Rpb2-(832)GYNQED(837), Rpc128-(764)GYDIED(769)) and highly conserved in all cellular and viral DNA-dependent RNA polymerases. A site-directed mutagenesis of this dicarboxylic motif reveals its strictly essential character in RNA polymerase III, with a slightly less stringent pattern in RNA polymerase II, where ... [more]

Unknown Jul. 15, 2011; 0(0); [Pubmed: 21761155]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPO21 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Eyboulet F (2020)	High	-	BioGRID	-
RPB4 RPO21	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Harel-Sharvit L (2010)	High	-	BioGRID	502368
RPO21 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
RPO21 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
RPB4 RPO21	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kruk JA (2011)	Low	-	BioGRID	-
RPO21 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	McCann TS (2016)	Low	-	BioGRID	2202801
RPO21 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3605282
RPB4 RPO21	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Richard S (2021)	High	-	BioGRID	-
RPO21 RPB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Roether S (2010)	Low	-	BioGRID	-
RPB4 RPO21	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Babbarwal V (2014)	Low	-	BioGRID	-
RPB4 RPO21	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Goler-Baron V (2008)	Low	-	BioGRID	-
RPO21 RPB4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Leblanc BP (2000)	Low	-	BioGRID	-
RPO21 RPB4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Li W (2014)	Low	-	BioGRID	-
RPO21 RPB4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sheffer A (1999)	Low	-	BioGRID	-
RPO21 RPB4	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Kettenberger H (2004)	Low	-	BioGRID	-
RPO21 RPB4	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Otero G (1999)	Low	-	BioGRID	-
RPO21 RPB4	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Suh MH (2005)	Low	-	BioGRID	-
RPO21 RPB4	Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.	Aristizabal MJ (2013)	High	4.4101	BioGRID	3378110

Curated By

BioGRID

Interaction Summary

RPB4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA-directed RNA polymerase activity [IDA]single-stranded DNA binding [IDA]single-stranded RNA binding [IDA]translation initiation factor binding [IPI]

Gene Ontology Cellular Component

RPO21

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activity [IDA]RNA-directed RNA polymerase activity [IDA]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases.

Throughput

Ontology Terms

Related interactions

Curated By

RNA-directed RNA polymerase activity [IDA]
single-stranded DNA binding [IDA]
single-stranded RNA binding [IDA]
translation initiation factor binding [IPI]

RNA polymerase II activity [IDA]
RNA-directed RNA polymerase activity [IDA]