ZAP70
Gene Ontology Biological Process
- T cell receptor signaling pathway [IMP]
- beta selection [IGI]
- cell migration [IBA]
- immune response [ISO]
- innate immune response [IBA]
- intracellular signal transduction [IMP]
- negative thymic T cell selection [IMP]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA, IMP, ISO]
- positive regulation of T cell differentiation [IMP, ISO]
- positive regulation of alpha-beta T cell differentiation [IGI, IMP]
- positive regulation of alpha-beta T cell proliferation [IGI]
- positive regulation of calcium-mediated signaling [IMP]
- positive thymic T cell selection [IMP, ISO]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA, ISO]
- thymic T cell selection [IMP]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LCK
Gene Ontology Biological Process
- B cell receptor signaling pathway [IDA]
- T cell differentiation [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- cell surface receptor signaling pathway [IDA]
- cellular response to peptide hormone stimulus [IBA]
- dephosphorylation [ISO]
- innate immune response [IBA]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [IDA, IMP, ISO]
- positive regulation of T cell activation [ISO]
- positive regulation of T cell receptor signaling pathway [IBA]
- positive regulation of gamma-delta T cell differentiation [IMP]
- positive regulation of gene expression [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [ISO]
- positive regulation of tyrosine phosphorylation of Stat5 protein [IDA]
- positive regulation of uterine smooth muscle contraction [ISO]
- protein autophosphorylation [IDA, ISO]
- protein phosphorylation [IDA, ISO]
- regulation of T cell receptor signaling pathway [IDA]
- regulation of apoptotic process [IBA]
- regulation of cell proliferation [IBA]
- release of sequestered calcium ion into cytosol [IDA]
- response to drug [ISO]
- response to hydrogen peroxide [ISO]
- response to mechanical stimulus [ISO]
- response to metal ion [ISO]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function- ATPase binding [ISO]
- CD4 receptor binding [ISO]
- CD8 receptor binding [ISO]
- SH2 domain binding [ISO]
- antigen binding [ISO]
- glycoprotein binding [ISO]
- identical protein binding [ISO]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphatidylinositol 3-kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- protein serine/threonine phosphatase activity [ISO]
- protein tyrosine kinase activity [IDA, IMP, ISO]
- receptor binding [IBA]
- ATPase binding [ISO]
- CD4 receptor binding [ISO]
- CD8 receptor binding [ISO]
- SH2 domain binding [ISO]
- antigen binding [ISO]
- glycoprotein binding [ISO]
- identical protein binding [ISO]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphatidylinositol 3-kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- protein serine/threonine phosphatase activity [ISO]
- protein tyrosine kinase activity [IDA, IMP, ISO]
- receptor binding [IBA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Regulation of T cell development by the deubiquitinating enzyme CYLD.
T cell receptor signaling is essential for the generation and maturation of T lymphocyte precursors. Here we identify the deubiquitinating enzyme CYLD as a positive regulator of proximal T cell receptor signaling in thymocytes. CYLD physically interacted with active Lck and promoted recruitment of active Lck to its substrate, Zap70. CYLD also removed both Lys 48- and Lys 63-linked polyubiquitin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ZAP70 LCK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| LCK ZAP70 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID