BAIT
GATA4
Gata-4
GATA binding protein 4
GO Process (66)
GO Function (17)
GO Component (3)
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- SMAD protein signal transduction [IDA]
- Sertoli cell differentiation [IMP]
- atrial septum morphogenesis [ISO]
- atrial septum primum morphogenesis [IMP]
- atrial septum secundum morphogenesis [ISO]
- atrioventricular canal development [IGI]
- atrioventricular valve formation [IGI]
- atrioventricular valve morphogenesis [IGI, TAS]
- canonical Wnt signaling pathway [IDA]
- cardiac muscle cell differentiation [IGI, IMP, NAS]
- cardiac muscle hypertrophy in response to stress [IMP]
- cardiac muscle tissue development [IGI]
- cardiac right ventricle morphogenesis [IMP]
- cardiac septum development [IGI]
- cell growth involved in cardiac muscle cell development [ISO]
- cell-cell signaling [IMP]
- cellular response to follicle-stimulating hormone stimulus [IDA]
- cellular response to gonadotropin stimulus [IDA]
- diaphragm morphogenesis [TAS]
- embryonic digestive tract morphogenesis [IMP]
- embryonic foregut morphogenesis [IMP]
- embryonic heart tube anterior/posterior pattern specification [IMP]
- embryonic heart tube development [IMP]
- embryonic morphogenesis [IMP]
- endocardial cushion development [IGI, IMP, ISO]
- endoderm formation [IMP]
- epithelial cell fate commitment [IMP]
- gastrulation with mouth forming second [IMP]
- heart development [IMP]
- heart looping [IMP]
- heart morphogenesis [IMP]
- in utero embryonic development [IMP]
- intestinal epithelial cell differentiation [ISO]
- lung lobe formation [IMP]
- lung morphogenesis [TAS]
- male gonad development [ISO]
- mitral valve formation [TAS]
- negative regulation of autophagy [ISO]
- outflow tract morphogenesis [TAS]
- positive regulation of BMP signaling pathway [ISO]
- positive regulation of angiogenesis [IDA]
- positive regulation of cardiac muscle cell proliferation [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation vascular endothelial growth factor production [IDA]
- regulation of cardiac muscle cell contraction [ISO]
- regulation of cardiac muscle cell proliferation [IMP]
- regulation of gene expression [IDA, IGI]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA, ISO, TAS]
- response to drug [ISO]
- response to estrogen [IDA]
- response to mechanical stimulus [ISO]
- response to retinoic acid [IDA]
- seminiferous tubule development [IMP]
- signal transduction involved in regulation of gene expression [IDA]
- skeletal muscle cell differentiation [NAS]
- spermatogenesis [IMP]
- transcription from RNA polymerase II promoter [IDA, ISO]
- transdifferentiation [ISO]
- tricuspid valve formation [TAS]
- tube morphogenesis [IGI]
- vasculogenesis involved in coronary vascular morphogenesis [TAS]
- ventricular cardiac muscle tissue development [IGI]
- ventricular septum development [IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- NFAT protein binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- co-SMAD binding [ISO]
- enhancer sequence-specific DNA binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription coactivator activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA, ISO]
- NFAT protein binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- co-SMAD binding [ISO]
- enhancer sequence-specific DNA binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription coactivator activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
ACTB
BRWS1, PS1TP5BP1
actin, beta
GO Process (17)
GO Function (8)
GO Component (15)
Gene Ontology Biological Process
- 'de novo' posttranslational protein folding [TAS]
- ATP-dependent chromatin remodeling [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- adherens junction organization [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- cellular component movement [TAS]
- cellular protein metabolic process [TAS]
- chromatin organization [TAS]
- innate immune response [TAS]
- membrane organization [TAS]
- platelet aggregation [IMP]
- protein folding [TAS]
- retina homeostasis [IEP]
- substantia nigra development [IEP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
Gene Ontology Cellular Component
- MLL5-L complex [IDA]
- NuA4 histone acetyltransferase complex [IDA]
- blood microparticle [IDA]
- cytoplasm [TAS]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytoskeleton [TAS]
- cytosol [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- nuclear chromatin [IDA]
- nucleoplasm [TAS]
- protein complex [IDA]
- ribonucleoprotein complex [IDA]
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Cyclin-dependent kinase-9 is a component of the p300/GATA4 complex required for phenylephrine-induced hypertrophy in cardiomyocytes.
A zinc finger protein GATA4 is one of the hypertrophy-responsive transcription factors and forms a complex with an intrinsic histone acetyltransferase, p300. Disruption of this complex results in the inhibition of cardiomyocyte hypertrophy and heart failure in vivo. By tandem affinity purification and mass spectrometric analyses, we identified cyclin-dependent kinase-9 (Cdk9) as a novel GATA4-binding partner. Cdk9 also formed a ... [more]
J. Biol. Chem. Mar. 26, 2010; 285(13);9556-68 [Pubmed: 20081228]
Throughput
- Low Throughput
Curated By
- BioGRID