BAIT
EP300
E1A binding protein p300
GO Process (78)
GO Function (34)
GO Component (7)
Gene Ontology Biological Process
- N-terminal peptidyl-lysine acetylation [ISO]
- apoptotic process [ISO]
- cellular response to antibiotic [IEP]
- cellular response to cAMP [IEP]
- cellular response to dexamethasone stimulus [IEP]
- cellular response to drug [IEP]
- cellular response to glucose stimulus [IEP]
- cellular response to hydrogen peroxide [IEP]
- cellular response to mineralocorticoid stimulus [IEP]
- cellular response to nerve growth factor stimulus [IEP]
- cellular response to organic cyclic compound [IEP]
- cellular response to retinoic acid [IEP]
- cellular response to trichostatin A [IEP]
- circadian rhythm [ISO]
- digestive tract development [IEP]
- heart development [ISO]
- histone H2B acetylation [ISO]
- histone H3 acetylation [IMP]
- histone H4 acetylation [ISO]
- histone acetylation [IMP]
- internal peptidyl-lysine acetylation [IMP, ISO]
- internal protein amino acid acetylation [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [ISO]
- liver development [IEP]
- lung development [ISO]
- memory [IEP]
- negative regulation of cell death [IMP]
- negative regulation of cellular metabolic process [IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of miRNA metabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- organ morphogenesis [ISO]
- positive regulation by host of viral transcription [ISO]
- positive regulation of DNA binding [IMP]
- positive regulation of axon extension [IMP]
- positive regulation of cell death [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell size [IMP]
- positive regulation of cellular metabolic process [IMP]
- positive regulation of collagen biosynthetic process [IMP]
- positive regulation of gene expression [IDA, IMP]
- positive regulation of glycoprotein biosynthetic process [IMP]
- positive regulation of histone acetylation [IMP]
- positive regulation of muscle atrophy [IMP]
- positive regulation of protein acetylation [IMP]
- positive regulation of protein binding [ISO]
- positive regulation of protein import into nucleus, translocation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of protein secretion [IMP]
- positive regulation of proteolysis [IMP]
- positive regulation of sarcomere organization [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of translation [IMP]
- protein kinase B signaling [IDA]
- protein-DNA complex assembly [IDA]
- regulation of androgen receptor signaling pathway [ISO]
- regulation of angiotensin metabolic process [IDA]
- regulation of nucleic acid-templated transcription [ISO]
- regulation of transcription, DNA-templated [ISO]
- regulation of tubulin deacetylation [ISO]
- response to calcium ion [IMP]
- response to cobalt ion [IEP]
- response to dexamethasone [IMP]
- response to drug [IMP]
- response to estrogen [IEP, ISO]
- response to ethanol [IEP]
- response to fatty acid [IEP]
- response to glucose [IMP]
- response to hydrogen peroxide [IEP]
- response to hypoxia [IEP, ISO]
- response to organic cyclic compound [IEP]
- response to retinoic acid [IEP]
- response to tumor necrosis factor [IEP]
- skeletal muscle tissue development [ISO]
- somitogenesis [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [ISO]
- NF-kappaB binding [IPI]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- SMAD binding [IPI]
- acetyltransferase activity [ISO]
- activating transcription factor binding [IPI, ISO]
- androgen receptor binding [ISO]
- antigen binding [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [ISO]
- chromatin DNA binding [IDA, ISO]
- chromatin binding [IDA, ISO]
- core promoter binding [IDA, ISO]
- core promoter proximal region DNA binding [IDA]
- glucocorticoid receptor binding [IDA]
- histone acetyltransferase activity [IDA, ISO]
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IMP, ISO]
- mitogen-activated protein kinase binding [IPI]
- nuclear hormone receptor binding [ISO]
- p53 binding [IPI, ISO]
- peroxisome proliferator activated receptor binding [IDA]
- pre-mRNA intronic binding [ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA, IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding transcription factor activity [IMP]
- transcription coactivator activity [ISO]
- transcription factor binding [IDA, IPI, ISO]
- transferase activity, transferring acyl groups [ISO]
- DNA binding [ISO]
- NF-kappaB binding [IPI]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- SMAD binding [IPI]
- acetyltransferase activity [ISO]
- activating transcription factor binding [IPI, ISO]
- androgen receptor binding [ISO]
- antigen binding [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [ISO]
- chromatin DNA binding [IDA, ISO]
- chromatin binding [IDA, ISO]
- core promoter binding [IDA, ISO]
- core promoter proximal region DNA binding [IDA]
- glucocorticoid receptor binding [IDA]
- histone acetyltransferase activity [IDA, ISO]
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IMP, ISO]
- mitogen-activated protein kinase binding [IPI]
- nuclear hormone receptor binding [ISO]
- p53 binding [IPI, ISO]
- peroxisome proliferator activated receptor binding [IDA]
- pre-mRNA intronic binding [ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA, IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding transcription factor activity [IMP]
- transcription coactivator activity [ISO]
- transcription factor binding [IDA, IPI, ISO]
- transferase activity, transferring acyl groups [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
GATA4
GATA binding protein 4
GO Process (61)
GO Function (17)
GO Component (3)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- SMAD protein signal transduction [ISO]
- Sertoli cell differentiation [ISO]
- atrial septum morphogenesis [ISO]
- atrial septum primum morphogenesis [ISO]
- atrial septum secundum morphogenesis [ISO]
- atrioventricular canal development [ISO]
- atrioventricular valve formation [ISO]
- atrioventricular valve morphogenesis [ISO]
- canonical Wnt signaling pathway [ISO]
- cardiac muscle cell differentiation [IEP, ISO]
- cardiac muscle hypertrophy in response to stress [ISO]
- cardiac muscle tissue development [ISO]
- cardiac right ventricle morphogenesis [ISO]
- cardiac septum development [ISO]
- cell growth involved in cardiac muscle cell development [IDA]
- cell-cell signaling [ISO]
- cellular response to follicle-stimulating hormone stimulus [ISO]
- cellular response to glucose stimulus [IEP]
- cellular response to gonadotropin stimulus [ISO]
- embryonic digestive tract morphogenesis [ISO]
- embryonic foregut morphogenesis [ISO]
- embryonic heart tube anterior/posterior pattern specification [ISO]
- embryonic heart tube development [ISO]
- embryonic morphogenesis [ISO]
- endocardial cushion development [ISO]
- endoderm formation [ISO]
- epithelial cell fate commitment [ISO]
- gastrulation with mouth forming second [ISO]
- heart development [ISO, TAS]
- heart looping [ISO]
- heart morphogenesis [ISO]
- in utero embryonic development [ISO]
- intestinal epithelial cell differentiation [ISO]
- lung lobe formation [ISO]
- male gonad development [ISO]
- negative regulation of autophagy [IMP]
- positive regulation of BMP signaling pathway [IDA]
- positive regulation of angiogenesis [ISO]
- positive regulation of cardiac muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation vascular endothelial growth factor production [ISO]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of cardiac muscle cell proliferation [ISO]
- regulation of gene expression [ISO]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- response to drug [ISO]
- response to estrogen [ISO]
- response to mechanical stimulus [IDA]
- response to retinoic acid [ISO]
- response to vitamin A [IEP]
- seminiferous tubule development [ISO]
- signal transduction involved in regulation of gene expression [ISO]
- spermatogenesis [ISO]
- transcription from RNA polymerase II promoter [ISO]
- transdifferentiation [IMP]
- tube morphogenesis [ISO]
- ventricular cardiac muscle tissue development [ISO]
- ventricular septum development [ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- NFAT protein binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity [ISO]
- activating transcription factor binding [IPI]
- chromatin binding [ISO]
- co-SMAD binding [ISO]
- enhancer sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IMP, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription coactivator activity [ISO]
- transcription factor binding [IDA, IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- zinc ion binding [TAS]
- DNA binding [IDA, ISO]
- NFAT protein binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity [ISO]
- activating transcription factor binding [IPI]
- chromatin binding [ISO]
- co-SMAD binding [ISO]
- enhancer sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IMP, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription coactivator activity [ISO]
- transcription factor binding [IDA, IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
Rattus norvegicus
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Cyclin-dependent kinase-9 is a component of the p300/GATA4 complex required for phenylephrine-induced hypertrophy in cardiomyocytes.
A zinc finger protein GATA4 is one of the hypertrophy-responsive transcription factors and forms a complex with an intrinsic histone acetyltransferase, p300. Disruption of this complex results in the inhibition of cardiomyocyte hypertrophy and heart failure in vivo. By tandem affinity purification and mass spectrometric analyses, we identified cyclin-dependent kinase-9 (Cdk9) as a novel GATA4-binding partner. Cdk9 also formed a ... [more]
J. Biol. Chem. Mar. 26, 2010; 285(13);9556-68 [Pubmed: 20081228]
Throughput
- Low Throughput
Additional Notes
- ChIP
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GATA4 EP300 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1055911 |
Curated By
- BioGRID