Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.

Drane P, Ouararhni K, Depaux A, Shuaib M, Hamiche A

The histone variant H3.3 marks active chromatin by replacing the conventional histone H3.1. In this study, we investigate the detailed mechanism of H3.3 replication-independent deposition. We found that the death domain-associated protein DAXX and the chromatin remodeling factor ATRX (alpha-thalassemia/mental retardation syndrome protein) are specifically associated with the H3.3 deposition machinery. Bacterially expressed DAXX has a marked binding preference for ... [more]

Genes Dev. Jun. 15, 2010; 24(12);1253-65 [Pubmed: 20504901]

Throughput

  • Low Throughput

Curated By

  • BioGRID