Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The protein network surrounding the human telomere repeat binding factors TRF1, TRF2, and POT1.

Giannone RJ, McDonald HW, Hurst GB, Shen RF, Wang Y, Liu Y

Telomere integrity (including telomere length and capping) is critical in overall genomic stability. Telomere repeat binding factors and their associated proteins play vital roles in telomere length regulation and end protection. In this study, we explore the protein network surrounding telomere repeat binding factors, TRF1, TRF2, and POT1 using dual-tag affinity purification in combination with multidimensional protein identification technology liquid ... [more]

PLoS ONE Sep. 03, 2010; 5(8);e12407 [Pubmed: 20811636]

Throughput

  • Low Throughput

Curated By

  • BioGRID