BAIT

TIF1

translation initiation factor eIF4A, eIF4A, L000002302, YKR059W

Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication

GO Process (2)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

regulation of translational initiation [ISS]

translational initiation [IDA, IMP]

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IDA, IMP]
RNA-dependent ATPase activity [IDA]
translation initiation factor activity [IDA, IMP, ISS, TAS]

Gene Ontology Cellular Component

cytoplasm [ISS]

eukaryotic translation initiation factor 4F complex [IGI, ISS]

ribosome [TAS]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TIF4631

eiF4G1, L000002309, YGR162W

Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication

GO Process (3)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

ribosomal large subunit biogenesis [IMP]

stress granule assembly [IMP]

translational initiation [IMP]

Gene Ontology Molecular Function

RNA binding [IDA]
mRNA binding [IDA]
translation initiation factor activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

cytoplasmic stress granule [IDA]

eukaryotic translation initiation factor 4F complex [IGI, IMP, ISS]

mitochondrion [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S pre-initiation complex accumulation, while the pentose phosphate pathway is co-ordinately up-regulated.

Castelli LM, Lui J, Campbell SG, Rowe W, Zeef LA, Holmes LE, Hoyle NP, Bone J, Selley JN, Sims PF, Ashe MP

Cellular stress can globally inhibit translation initiation and glucose removal from yeast causes one of the most dramatic effects in terms of rapidity and scale. Here we show that the same rapid inhibition occurs during yeast growth as glucose levels diminish. We characterise this novel regulation showing that it involves alterations within the 48S pre-initiation complex. In particular, the interaction ... [more]

Unknown Jul. 27, 2011; 0(0); [Pubmed: 21795399]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TIF4631 TIF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
TIF1 TIF4631	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
TIF1 TIF4631	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	4	BioGRID	3605095
TIF4631 TIF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Berset C (2003)	Low	-	BioGRID	-
TIF4631 TIF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Castelli LM (2011)	Low	-	BioGRID	-
TIF4631 TIF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Chang Y (2018)	Low	-	BioGRID	-
TIF4631 TIF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Dominguez D (1999)	Low	-	BioGRID	-
TIF1 TIF4631	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Haimov O (2018)	Low	-	BioGRID	-
TIF4631 TIF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Park EH (2013)	Low	-	BioGRID	856471
TIF4631 TIF1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Schuetz P (2008)	Low	-	BioGRID	331073
TIF4631 TIF1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Mohammad MP (2021)	Low	-	BioGRID	3308648
TIF4631 TIF1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Wilmes GM (2008)	High	-5.8003	BioGRID	309722
TIF1 TIF4631	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Andreou AZ (2017)	Low	-	BioGRID	-
TIF4631 TIF1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Dominguez D (1999)	Low	-	BioGRID	-
TIF1 TIF4631	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Neff CL (1999)	Low	-	BioGRID	-
TIF1 TIF4631	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Schuetz P (2008)	Low	-	BioGRID	331075
TIF4631 TIF1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Singh CR (2012)	Low	-	BioGRID	-
TIF4631 TIF1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	de la Cruz J (1997)	Low	-	BioGRID	163606
TIF4631 TIF1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Park EH (2013)	Low	-	BioGRID	856466

Curated By

BioGRID

Interaction Summary

TIF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IDA, IMP]RNA-dependent ATPase activity [IDA]translation initiation factor activity [IDA, IMP, ISS, TAS]

Gene Ontology Cellular Component

TIF4631

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]mRNA binding [IDA]translation initiation factor activity [TAS]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S pre-initiation complex accumulation, while the pentose phosphate pathway is co-ordinately up-regulated.

Throughput

Related interactions

Curated By

ATP-dependent RNA helicase activity [IDA, IMP]
RNA-dependent ATPase activity [IDA]
translation initiation factor activity [IDA, IMP, ISS, TAS]

RNA binding [IDA]
mRNA binding [IDA]
translation initiation factor activity [TAS]