Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Ubiquitin ligase Hrd1 enhances the degradation and suppresses the toxicity of polyglutamine-expanded huntingtin.

Yang H, Zhong X, Ballar P, Luo S, Shen Y, Rubinsztein DC, Monteiro MJ, Fang S

E3 ubiquitin ligases catalyze the conjugation of ubiquitin onto proteins, which acts as a signal for targeting proteins for degradation by the proteasome. Hrd1 is an endoplasmic reticulum (ER) membrane-spanning E3 with its catalytic active RING finger facing the cytosol. We speculated that this topology might allow Hrd1 to ubiquitinate misfolded proteins in the cytosol. We tested this idea by ... [more]

Exp. Cell Res. Feb. 01, 2007; 313(3);538-50 [Pubmed: 17141218]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: abnormal cell death (MP:0000313)

Additional Notes

  • Overexpression of Hrd1 partially rescues the lethality of Htt-polyglutamine expansion mutants

Curated By

  • BioGRID