BTRC
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IBA]
- branching involved in mammary gland duct morphogenesis [IMP]
- cellular response to organic cyclic compound [IDA]
- mammary gland epithelial cell proliferation [IMP]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of circadian rhythm [IMP]
- positive regulation of proteolysis [ISO]
- positive regulation of transcription, DNA-templated [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein catabolic process [IMP]
- protein dephosphorylation [IDA]
- protein destabilization [ISO]
- protein polyubiquitination [IDA]
- protein ubiquitination [ISO]
- regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- regulation of cell cycle [IMP]
- regulation of circadian rhythm [ISO]
- regulation of proteasomal protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NFKBIA
Gene Ontology Biological Process
- cytoplasmic sequestering of NF-kappaB [ISO]
- cytoplasmic sequestering of transcription factor [ISO]
- lipopolysaccharide-mediated signaling pathway [IDA]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of Notch signaling pathway [IMP]
- negative regulation of lipid storage [ISO]
- negative regulation of macrophage derived foam cell differentiation [ISO]
- negative regulation of myeloid cell differentiation [IMP]
- nucleotide-binding oligomerization domain containing 1 signaling pathway [IDA]
- nucleotide-binding oligomerization domain containing 2 signaling pathway [IDA]
- positive regulation of cellular protein metabolic process [ISO]
- positive regulation of cholesterol efflux [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [IMP]
- protein import into nucleus, translocation [IDA]
- regulation of cell proliferation [IDA]
- regulation of gene expression [IMP]
- response to exogenous dsRNA [IDA]
- response to lipopolysaccharide [IDA]
- response to muramyl dipeptide [IDA]
- response to muscle stretch [IDA]
- toll-like receptor 4 signaling pathway [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ubiquitin-dependent degradation of IkappaBalpha is mediated by a ubiquitin ligase Skp1/Cul 1/F-box protein FWD1.
Activation of the transcription factor nuclear factor kappa B (NF-kappaB) is controlled by proteolysis of its inhibitory subunit (IkappaB) via the ubiquitin-proteasome pathway. Signal-induced phosphorylation of IkappaBalpha by a large multisubunit complex containing IkappaB kinases is a prerequisite for ubiquitination. Here, we show that FWD1 (a mouse homologue of Slimb/betaTrCP), a member of the F-box/WD40-repeat proteins, is associated specifically with ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NFKBIA BTRC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 557327 | |
| NFKBIA BTRC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BTRC NFKBIA | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 611085 |
Curated By
- BioGRID