RRAGA
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- cell death [IDA]
- cellular protein localization [IMP]
- cellular response to amino acid stimulus [IMP]
- cellular response to starvation [IBA]
- modulation by virus of host morphology or physiology [IDA]
- positive regulation of TOR signaling [IBA, NAS]
- positive regulation of cytolysis [IDA]
- regulation of autophagy [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LAMTOR1
Gene Ontology Biological Process
- cell growth [IMP]
- cellular protein localization [IMP]
- cellular response to amino acid stimulus [IMP]
- cholesterol homeostasis [IMP]
- endosome localization [ISS]
- endosome organization [ISS]
- lysosome localization [ISS]
- lysosome organization [ISS]
- positive regulation of GTPase activity [IDA]
- positive regulation of MAPK cascade [ISS]
- positive regulation of TOR signaling [IMP]
- regulation of cholesterol efflux [IMP]
- regulation of cholesterol esterification [IMP]
- regulation of cholesterol import [IMP]
- regulation of receptor recycling [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Crystal structure of the Gtr1p-Gtr2p complex reveals new insights into the amino acid-induced TORC1 activation.
The target of rapamycin (TOR) complex 1 (TORC1) is a central cell growth regulator in response to a wide array of signals. The Rag GTPases play an essential role in relaying amino acid signals to TORC1 activation through direct interaction with raptor and recruitment of the TORC1 complex to lysosomes. Here we present the crystal structure of the Gtr1p-Gtr2p complex, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LAMTOR1 RRAGA | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3166904 | |
| RRAGA LAMTOR1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| LAMTOR1 RRAGA | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 500 | BioGRID | 2993614 |
Curated By
- BioGRID