BAIT
H2AFX
H2A.X, H2A/X, H2AX
H2A histone family, member X
GO Process (8)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SOD2
IPOB, MNSOD, MVCD6, RP1-56L9.2
superoxide dismutase 2, mitochondrial
GO Process (13)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- age-dependent response to reactive oxygen species [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of neuron apoptotic process [IGI]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- oxygen homeostasis [IMP]
- protein homotetramerization [IPI]
- regulation of blood pressure [ISS]
- regulation of transcription from RNA polymerase II promoter [IMP]
- release of cytochrome c from mitochondria [ISS]
- removal of superoxide radicals [IMP]
- response to superoxide [IMP]
- superoxide metabolic process [IDA]
- vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.
The replacement histone variant H2AX senses DNA double-strand breaks (DSBs) and recruits characteristic sets of proteins at its phosphorylated (gamma-H2AX) foci for concurrent DNA repair. We reasoned that the H2AX interaction network, or interactome, formed in the tumor-associated DNA DSB environment such as in hepatocellular carcinoma (HCC) cells, where preneoplastic lesions frequently occur, is indicative of HCC pathogenic status. By ... [more]
J. Proteome Res. Mar. 05, 2010; 9(3);1402-15 [Pubmed: 20000738]
Throughput
- Low Throughput
Ontology Terms
- hepatoma cell line (BTO:0000578) [hepatocellular carcinoma (DOID:684)]
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID