Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.

Yang X, Zou P, Yao J, Yun D, Bao H, Du R, Long J, Chen X

The replacement histone variant H2AX senses DNA double-strand breaks (DSBs) and recruits characteristic sets of proteins at its phosphorylated (gamma-H2AX) foci for concurrent DNA repair. We reasoned that the H2AX interaction network, or interactome, formed in the tumor-associated DNA DSB environment such as in hepatocellular carcinoma (HCC) cells, where preneoplastic lesions frequently occur, is indicative of HCC pathogenic status. By ... [more]

J. Proteome Res. Mar. 05, 2010; 9(3);1402-15 [Pubmed: 20000738]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: hepatoma cell line (BTO:0000578) [hepatocellular carcinoma (DOID:684)]

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID