BAIT

CUL5

VACM-1, VACM1
cullin 5
UPS Project
Homo sapiens
PREY

COMMD1

C2orf5, MURR1
copper metabolism (Murr1) domain containing 1
UPS Project
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Copper metabolism MURR1 domain containing 1 (COMMD1) regulates Cullin-RING ligases by preventing Cullin-associated NEDD8-dissociated (CAND1) binding.

Mao X, Gluck N, Chen B, Starokadomskyy P, Li H, Maine GN, Burstein E

Cullin-RING ligases (CRLs), the most prolific class of ubiquitin ligase enzymes, are multimeric complexes that regulate multiple cellular processes. CRL activity is regulated by Cullin-associated Nedd8-dissociated protein 1 (CAND1), an inhibitor that promotes the dissociation of substrate receptor components from the CRL. We demonstrate here that Copper metabolism MURR1 domain containing 1 (COMMD1), a factor previously found to promote ubiquitination ... [more]

Unknown Jul. 21, 2011; 0(0); [Pubmed: 21778237]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
COMMD1 CUL5
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID