STUB1
Gene Ontology Biological Process
- cellular response to misfolded protein [IDA]
- misfolded or incompletely synthesized protein catabolic process [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of chaperone-mediated protein complex assembly [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein maturation [TAS]
- protein polyubiquitination [IDA, IMP]
- regulation of glucocorticoid metabolic process [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
Gene Ontology Cellular Component
SOD1
Gene Ontology Biological Process
- activation of MAPK activity [ISS]
- anterograde axon cargo transport [ISS]
- auditory receptor cell stereocilium organization [ISS]
- blood coagulation [TAS]
- cell aging [IMP]
- cellular iron ion homeostasis [ISS]
- embryo implantation [ISS, NAS]
- glutathione metabolic process [ISS]
- heart contraction [IDA]
- hydrogen peroxide biosynthetic process [IDA, ISS]
- locomotory behavior [ISS]
- muscle cell cellular homeostasis [ISS]
- myeloid cell homeostasis [ISS]
- negative regulation of cholesterol biosynthetic process [IDA]
- negative regulation of neuron apoptotic process [ISS]
- neurofilament cytoskeleton organization [ISS]
- ovarian follicle development [ISS]
- peripheral nervous system myelin maintenance [ISS]
- placenta development [NAS]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of apoptotic process [IC]
- positive regulation of catalytic activity [IDA]
- positive regulation of cytokine production [IDA]
- positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- positive regulation of superoxide anion generation [IDA]
- reactive oxygen species metabolic process [IDA]
- regulation of Rac GTPase activity [IDA]
- regulation of T cell differentiation in thymus [NAS]
- regulation of blood pressure [ISS]
- regulation of mitochondrial membrane potential [IMP]
- regulation of multicellular organism growth [ISS]
- regulation of organ growth [NAS]
- regulation of protein kinase activity [IDA]
- relaxation of vascular smooth muscle [ISS]
- removal of superoxide radicals [IBA, IC, ISS]
- response to axon injury [ISS]
- response to drug [ISS]
- response to ethanol [ISS]
- response to heat [ISS]
- response to hydrogen peroxide [ISS]
- response to organic substance [IDA]
- response to superoxide [IDA]
- retina homeostasis [ISS]
- retrograde axon cargo transport [ISS]
- sensory perception of sound [ISS]
- spermatogenesis [ISS]
- superoxide metabolic process [IDA, ISS]
- thymus development [NAS]
- transmission of nerve impulse [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytosol [IDA, TAS]
- dendrite cytoplasm [IDA]
- extracellular matrix [IDA]
- extracellular region [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- mitochondrial intermembrane space [TAS]
- mitochondrial matrix [NAS]
- mitochondrion [IDA]
- neuronal cell body [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- peroxisome [IDA, ISS]
- plasma membrane [IDA]
- protein complex [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Dorfin-CHIP chimeric proteins potently ubiquitylate and degrade familial ALS-related mutant SOD1 proteins and reduce their cellular toxicity.
The ubiquitin-proteasome system (UPS) is involved in the pathogenetic mechanisms of neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Dorfin is a ubiquitin ligase (E3) that degrades mutant SOD1 proteins, which are responsible for familial ALS. Although Dorfin has potential as an anti-ALS molecule, its life in cells is short. To improve its stability and enhance its E3 activity, we developed ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SOD1 STUB1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID