ITPR3
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [ISO]
- calcium ion transport into cytosol [ISO, ISS]
- cellular response to cAMP [IDA]
- inositol phosphate-mediated signaling [IDA, ISO, ISS, TAS]
- long-term synaptic potentiation [ISO]
- memory [ISO]
- positive regulation of cytosolic calcium ion concentration [IDA]
- protein heterooligomerization [IDA]
- protein homooligomerization [IDA]
- response to calcium ion [ISO]
- sensory perception of bitter taste [ISO]
- sensory perception of sweet taste [ISO]
- sensory perception of umami taste [ISO]
Gene Ontology Molecular Function- calcium ion binding [IDA]
- calcium ion transmembrane transporter activity [TAS]
- inositol 1,3,4,5 tetrakisphosphate binding [IDA]
- inositol 1,4,5 trisphosphate binding [IDA, ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO, ISS, TAS]
- inositol hexakisphosphate binding [IDA]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- phosphatidylinositol binding [ISO]
- calcium ion binding [IDA]
- calcium ion transmembrane transporter activity [TAS]
- inositol 1,3,4,5 tetrakisphosphate binding [IDA]
- inositol 1,4,5 trisphosphate binding [IDA, ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO, ISS, TAS]
- inositol hexakisphosphate binding [IDA]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- phosphatidylinositol binding [ISO]
Gene Ontology Cellular Component
- apical part of cell [IDA]
- brush border [IDA]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- dendrite [IDA]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA, ISO, TAS]
- integral component of plasma membrane [ISO]
- membrane [ISO]
- myelin sheath [IDA]
- neuronal cell body [IDA]
- nuclear envelope [IDA]
- nuclear outer membrane [IDA]
- nucleolus [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [ISO]
- receptor complex [ISO]
UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Down-regulation of types I, II and III inositol 1,4,5-trisphosphate receptors is mediated by the ubiquitin/proteasome pathway.
Activation of certain phosphoinositidase-C-linked cell-surface receptors is known to cause an acceleration of the proteolysis of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] receptors and, thus, lead to Ins(1,4,5)P3-receptor down-regulation. In the current study we have sought to determine whether the ubiquitin/proteasome pathway is involved in this adaptive response. The data presented show (i) that activation of phosphoinositidase-C-linked receptors causes Ins(1,4,5)P3-receptor ubiquitination in a ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITPR3 UBC | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| UBC ITPR3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ITPR3 UBC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ITPR3 UBC | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID