BAIT

CDK5R1

CDK5P35, CDK5R, NCK5A, p23, p25, p35, p35nck5a
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A nestin scaffold links Cdk5/p35 signaling to oxidant-induced cell death.

Sahlgren CM, Pallari HM, He T, Chou YH, Goldman RD, Eriksson JE

The intermediate filament protein, nestin, has been implicated as an organizer of survival-determining signaling molecules. When nestin expression was related to the sensitivity of neural progenitor cells to oxidant-induced apoptosis, nestin displayed a distinct cytoprotective effect. Oxidative stress in neuronal precursor cells led to downregulation of nestin with subsequent activation of cyclin-dependent kinase 5 (Cdk5), a crucial kinase in the ... [more]

EMBO J. Oct. 18, 2006; 25(20);4808-19 [Pubmed: 17036052]

Throughput

  • Low Throughput

Curated By

  • BioGRID