BAIT

ARRB2

ARB2, ARR2, BARR2
arrestin, beta 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The N Terminus of the Adhesion G Protein-coupled Receptor GPR56 Controls Receptor Signaling Activity.

Paavola KJ, Stephenson JR, Ritter SL, Alter SP, Hall RA

GPR56 is an adhesion G protein-coupled receptor that plays a key role in cortical development. Mutations to GPR56 in humans cause malformations of the cerebral cortex, but little is known about the normal function of the receptor. We found that the large N terminus (NT) of GPR56 is cleaved from the rest of the receptor during processing but remains non-covalently ... [more]

J. Biol. Chem. Aug. 19, 2011; 286(33);28914-21 [Pubmed: 21708946]

Throughput

  • Low Throughput

Curated By

  • BioGRID