Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The SAM domains of Anks family proteins are critically involved in modulating the degradation of EphA receptors.

Kim J, Lee H, Kim Y, Yoo S, Park E, Park S

We recently reported that the phosphotyrosine-binding (PTB) domain of Anks family proteins binds to EphA8, thereby positively regulating EphA8-mediated signaling pathways. In the current study, we identified a potential role for the SAM domains of Anks family proteins in EphA signaling. We found that SAM domains of Anks family proteins directly bind to ubiquitin, suggesting that Anks proteins regulate the ... [more]

Mol. Cell. Biol. Apr. 01, 2010; 30(7);1582-92 [Pubmed: 20100865]

Throughput

  • Low Throughput

Additional Notes

  • AIDA-1 and ODIN show a preference for ubiquitinated EPHA8

Curated By

  • BioGRID