BAIT

SNR30

L000001968

H/ACA box small nucleolar RNA (snoRNA); essential gene required for cleavage of 35S primary rRNA transcript to release the precursor to the 18S rRNA

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

PREY

RPS18A

ribosomal 40S subunit protein S18A, S18A, S13, L000004480, YDR450W

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress

GO Process (3)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

cytoplasmic translation [NAS]

endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]

rRNA export from nucleus [IGI]

Gene Ontology Molecular Function

structural constituent of ribosome [NAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytosolic small ribosomal subunit [IBA, NAS]

mitochondrion [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

Identification of novel proteins associated with yeast snR30 small nucleolar RNA.

Lemay V, Hossain A, Osheim YN, Beyer AL, Dragon F

H/ACA small nucleolar RNPs (snoRNPs) that guide pseudouridylation reactions are comprised of one small nucleolar RNA (snoRNA) and four common proteins (Cbf5, Gar1, Nhp2 and Nop10). Unlike other H/ACA snoRNPs, snR30 is essential for the early processing reactions that lead to the production of 18S ribosomal RNA in the yeast Saccharomyces cerevisiae. To determine whether snR30 RNP contains specific proteins ... [more]

Unknown Sep. 05, 2011; 0(0); [Pubmed: 21893585]

Throughput

Low Throughput

Additional Notes

proteins were isolated by a two-step affinity purified protocol which used Gar1 as the bait in step one and snR30 as the bait in step 2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPS18A SNR30	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Lemay V (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary