BAIT

OSH2

oxysterol-binding protein related protein OSH2, YDL019C
Member of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH2 has a paralog, SWH1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

KES1

BSR3, LPI3, OSH4, oxysterol-binding protein KES1, L000000894, YPL145C
One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The Sterol-Binding Protein Kes1/Osh4p is a Regulator of Polarized Exocytosis.

Alfaro G, Johansen J, Dighe SA, Duamel G, Kozminski KG, Beh CT

Oxysterol-binding protein (OSBP)-related protein Kes1/Osh4p is implicated in nonvesicular sterol transfer between membranes in Saccharomyces cerevisiae. However, we found that Osh4p associated with exocytic vesicles that move from the mother cell into the bud, where Osh4p facilitated vesicle docking by the exocyst tethering complex at sites of polarized growth on the plasma membrane. Osh4p formed complexes with the small GTPases ... [more]

Unknown Aug. 05, 2011; 0(0); [Pubmed: 21819498]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide accumulation (APO:0000149)
  • phenotype: protein/peptide distribution (APO:0000209)

Additional Notes

  • deletion of osh4 causes protein aggregation and increased protein aggregate lifespan in a mutant deleted for all other osh genes
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KES1 OSH2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1463BioGRID
420010

Curated By

  • BioGRID