BAIT

WDR1

Aip1, D5Wsu185e, rede

WD repeat domain 1

GO Process (2)

GO Function (1)

GO Component (5)

Gene Ontology Biological Process

regulation of oligodendrocyte differentiation [ISO]

sensory perception of sound [ISA]

Gene Ontology Molecular Function

actin binding [ISA]

Gene Ontology Cellular Component

actin cytoskeleton [ISA]

actin filament [ISO]

cytoplasm [ISO]

extracellular vesicular exosome [ISO]

myelin sheath [IDA]

CRISPR Database MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

CDC73

8430414L16Rik, BC027756, C130030P16Rik, C81219, Hrpt2, AC175246.2

cell division cycle 73, Paf1/RNA polymerase II complex component

GO Process (17)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

cellular response to lipopolysaccharide [IDA]

endodermal cell fate commitment [IMP]

histone H2B ubiquitination [ISO]

histone monoubiquitination [ISO]

mRNA polyadenylation [ISO]

negative regulation of G1/S transition of mitotic cell cycle [ISO]

negative regulation of cell proliferation [ISO]

negative regulation of epithelial cell proliferation [ISO]

negative regulation of fibroblast proliferation [ISO]

negative regulation of myeloid cell differentiation [ISO]

negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]

positive regulation of Wnt signaling pathway [ISO]

positive regulation of mRNA 3'-end processing [ISO]

positive regulation of transcription elongation from RNA polymerase II promoter [ISO]

positive regulation of transcription from RNA polymerase II promoter [ISO]

protein destabilization [ISO]

stem cell maintenance [IMP]

Gene Ontology Molecular Function

RNA polymerase II core binding [ISO]
protein binding [IPI]

Gene Ontology Cellular Component

Cdc73/Paf1 complex [ISO]

CRISPR Database MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.

Ding L, Paszkowski-Rogacz M, Nitzsche A, Slabicki MM, Heninger AK, de Vries I, Kittler R, Junqueira M, Shevchenko A, Schulz H, Hubner N, Doss MX, Sachinidis A, Hescheler J, Iacone R, Anastassiadis K, Stewart AF, Pisabarro MT, Caldarelli A, Poser I, Theis M, Buchholz F

Pluripotent embryonic stem cells (ESCs) maintain self-renewal while ensuring a rapid response to differentiation cues. The identification of genes maintaining ESC identity is important to develop these cells for their potential therapeutic use. Here we report a genome-scale RNAi screen for a global survey of genes affecting ESC identity via alteration of Oct4 expression. Factors with the strongest effect on ... [more]

Cell Stem Cell May. 08, 2009; 4(5);403-15 [Pubmed: 19345177]

Throughput

Low Throughput

Curated By

BioGRID