GAL3
Gene Ontology Biological Process
Gene Ontology Molecular Function
GAL4
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II transcription factor binding [IDA, IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IGI, IMP, IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II transcription factor binding [IDA, IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IGI, IMP, IPI]
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Quantitative epistasis analysis and pathway inference from genetic interaction data.
Inferring regulatory and metabolic network models from quantitative genetic interaction data remains a major challenge in systems biology. Here, we present a novel quantitative model for interpreting epistasis within pathways responding to an external signal. The model provides the basis of an experimental method to determine the architecture of such pathways, and establishes a new set of rules to infer ... [more]
Quantitative Score
- -0.39 [SGA Score]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
Additional Notes
- An epistasis analysis was carried out to quantitatively score galactose-dependent genetic interactions based on fitness defects estimated from the growth rate of double versus single mutants. Significant genetic interactions were identified using a p-value threshold of 0.05.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GAL4 GAL3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID