BAIT

RAD54

XRS1, DNA-dependent ATPase RAD54, L000001574, YGL163C
DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

MAG1

MMS5, L000000976, YER142C
3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Systematic pathway analysis using high-resolution fitness profiling of combinatorial gene deletions.

St Onge RP, Mani R, Oh J, Proctor M, Fung E, Davis RW, Nislow C, Roth FP, Giaever G

Systematic genetic interaction studies have illuminated many cellular processes. Here we quantitatively examine genetic interactions among 26 Saccharomyces cerevisiae genes conferring resistance to the DNA-damaging agent methyl methanesulfonate (MMS), as determined by chemogenomic fitness profiling of pooled deletion strains. We constructed 650 double-deletion strains, corresponding to all pairings of these 26 deletions. The fitness of single- and double-deletion strains were ... [more]

Nat. Genet. Feb. 01, 2007; 39(2);199-206 [Pubmed: 17206143]

Quantitative Score

  • -0.170539 [SGA Score]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: growth in exponential phase (APO:0000310)

Additional Notes

  • Genetic interactions were quantitatively examined among 26 genes related to DNA repair. In the presence of methyl methanesulfonate (MMS), 45 were classified as alleviating interactions (significantly positive epsilon score) and 68 were classified as aggravating interactions (significantly negative epsilon score) with a P-value < 0.01.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MAG1 RAD54
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2573BioGRID
2604987

Curated By

  • BioGRID