BAIT

UBC

HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

HSD17B12

KAR, SDR12C1
hydroxysteroid (17-beta) dehydrogenase 12
GO Process (4)
GO Function (1)
GO Component (1)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Large-scale analysis of the human ubiquitin-related proteome.

Matsumoto M, Hatakeyama S, Oyamada K, Oda Y, Nishimura T, Nakayama KI

Protein ubiquitylation contributes to the regulation of many cellular processes including protein degradation, receptor internalization, and repair of DNA damage. We now present a comprehensive characterization of ubiquitin-conjugated and ubiquitin-associated proteins in human cells. The proteins were purified by immunoaffinity chromatography under denaturing or native conditions. They were then digested with trypsin, and the resulting peptides were analyzed by 2-D ... [more]

Proteomics Nov. 01, 2005; 5(16);4145-51 [Pubmed: 16196087]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293t cell (BTO:0002181)

Additional Notes

  • #LPPI
  • Likely protein-protein interaction
  • native only

Curated By

  • BioGRID