ZNF281
Gene Ontology Biological Process
- embryonic body morphogenesis [ISS]
- negative regulation of gene expression [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- stem cell differentiation [ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- core promoter binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [ISS]
- transcription regulatory region DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- core promoter binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [ISS]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
NANOG
Gene Ontology Biological Process
- cell differentiation [IEP]
- cell proliferation [IMP]
- endodermal cell fate specification [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of cell differentiation [IMP]
- regulation of gene expression [IMP]
- regulation of transcription, DNA-templated [IDA]
- somatic stem cell maintenance [IMP]
- stem cell maintenance [IEP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The transcription factor Zfp281 controls embryonic stem cell pluripotency by direct activation and repression of target genes.
Oct4, Sox2, and Nanog are key components of a core transcriptional regulatory network that controls the ability of embryonic stem cells to differentiate into all cell types. Here we show that Zfp281, a zinc finger transcription factor, is a key component of the network and that it is required to maintain pluripotency. Zfp281 was shown to directly activate Nanog expression ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NANOG ZNF281 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID