SHARPIN
Gene Ontology Biological Process
- apoptotic nuclear changes [IMP]
- epidermis development [IMP]
- keratinization [IMP]
- mitochondrion organization [IMP]
- negative regulation of inflammatory response [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP, ISO]
- protein homooligomerization [ISO]
- protein linear polyubiquitination [ISO]
- regulation of CD40 signaling pathway [ISO]
- regulation of tumor necrosis factor-mediated signaling pathway [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RBCK1
Gene Ontology Biological Process
- T cell receptor signaling pathway [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of necroptotic process [IGI]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP, ISO]
- positive regulation of NF-kappaB import into nucleus [IMP]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of apoptotic process [IMP]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein linear polyubiquitination [ISO]
- protein polyubiquitination [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SHARPIN forms a linear ubiquitin ligase complex regulating NF-κB activity and apoptosis.
SHARPIN is a ubiquitin-binding and ubiquitin-like-domain-containing protein which, when mutated in mice, results in immune system disorders and multi-organ inflammation. Here we report that SHARPIN functions as a novel component of the linear ubiquitin chain assembly complex (LUBAC) and that the absence of SHARPIN causes dysregulation of NF-κB and apoptotic signalling pathways, explaining the severe phenotypes displayed by chronic proliferative ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RBCK1 SHARPIN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 2562939 | |
| SHARPIN RBCK1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 2562940 | |
| RBCK1 SHARPIN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RBCK1 SHARPIN | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2562941 |
Curated By
- BioGRID