BAIT

GRX4

monothiol glutaredoxin GRX4, S000007445, YER174C
Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; with Grx3p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; mutant has increased aneuploidy tolerance; transcription regulated by Yap5p; GRX4 has a paralog, GRX3, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

DRE2

YKR071C
Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2: requirement of Rnr4 and contribution of Grx3/4 and Dre2.

Zhang Y, Liu L, Wu X, An X, Stubbe J, Huang M

The β2 subunit of class Ia ribonucleotide reductase (RNR) contains a diferric tyrosyl radical cofactor (FeIII2-Y·) that is essential for nucleotide reduction. The β2 subunit of Saccharomyces cerevisiae is a heterodimer of Rnr2 (β) and Rnr4 (β'). Although only β is capable of iron binding and Y· formation, cells lacking β' are either dead or exhibit extremely low Y· levels ... [more]

Unknown Sep. 20, 2011; 0(0); [Pubmed: 21931161]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • genetic complex
  • loss of Dre2 causes growth defects in a Grx3/Grx4 mutant background

Curated By

  • BioGRID