BAIT

SPB4

L000001991, YFL002C
Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RPB5

DNA-directed RNA polymerase core subunit RPB5, ABC27, L000001590, L000001679, YBR154C
RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dynamics of the putative RNA helicase Spb4 during ribosome assembly in Saccharomyces cerevisiae.

Garcia-Gomez JJ, Lebaron S, Froment C, Monsarrat B, Henry Y, de la Cruz J

Spb4 is a putative ATP-dependent RNA helicase required for proper processing of 27SB pre-rRNAs and therefore for 60S ribosomal subunit biogenesis. To define the timing of association of this protein with pre-ribosomal particles, we have studied the composition of complexes that copurify with TAP-tagged Spb4. These complexes contain mainly the 27SB pre-rRNAs and about 50 ribosome biogenesis proteins, primarily components ... [more]

Unknown Aug. 08, 2011; 0(0); [Pubmed: 21825077]

Throughput

  • High Throughput

Additional Notes

  • factors associated with Spb4-TAP or dominant negative Spb4(R360A)-TAP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPB4 RPB5
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2169BioGRID
1930675

Curated By

  • BioGRID